ONGOING EXPERIENCE WITH FIELD INVERSION GEL ELECTROPHORESIS (FIGE) AS A TYPING SYSTEM FOR GROUP A STREPTOCOCCUS (GAS). † 1058
نویسندگان
چکیده
منابع مشابه
Field inversion gel electrophoresis as a typing system for group A streptococcus.
Field inversion gel electrophoresis (FIGE) was performed on 139 isolates of group A streptococcus (GAS) representing 72 different M types. Multiple isolates were tested for 27 M types (range, 2-19). Each of the M types tested had at least one unique banding pattern that was not observed in any other M type. For 23 of 27 M types for which multiple isolates were available, band profiles of the is...
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A rapid protocol for subtyping vancomycin-resistant enterococci by pulsed-field gel electrophoresis is reported. The procedure is simple and potentially cost-effective and allows reproducible subtyping of the strains in approximately 1 day.
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Pulsed field gel electrophoresis (PFGE) is capable of resolving large DNA molecules of up to two million nucleotides and is becoming a widely used technique in DNA studies (1—3). Three factors can potentially limit PFGE in a laboratory: 1. the cost of the apparatus, 2. reproducibility of electrophoretic conditions, and 3. unusually long electrophoretic time. To alleviate some of these problems ...
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We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise mo...
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Hiroko WAKIMOTO1)2), Masamichi OGURA2), Hisako YANO2), Akira KOGUCHI3), Yukio WAKIMOTO4), Souhei MIYAKAWA5), Matsuko Katsuko OKUZUMI6) & Masashi MIZOKAMI4) 1) Aichi Medical University School of Nursing 2) Nagoya City University Graduate School of Nursing 3) Abbott Japan 4) Nagoya City University Hospital 5) Kariwa-gun General Hospital 6) Dokkyo Medical University Hospital (Received November 2, ...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1996
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-199604001-01080